Microbiology Lab Manual for Face-to-Face Labs

This is an online, free lab manual for the student in BIOL 2310L.

Lab Units

Unit 1

Unit 2

Unit 3

• Sterilization of an Inoculating Tool using a Microincinerator (Video)
• Sterilization of an Inoculating Tool using a Bunsen Burner (Video)
• Transferring Organism from a Slant Medium to a Broth Medium (Video)
• Transferring Organism from a Petri dish to a Slant (Video)
• Transferring Organism from a Broth to a Deep (Video)
• Creating a Lawn Petri Plate (Environmental Sample Example) (Video)
• Inoculating a Petri Plate with the Streak Isolation Technique (Video)
• Carbohydrate Fermentation - Streak Isolation Results (Video)

Unit 4

• Negative Stain Procedure (Video)
• Single Specimen Slide (Video)
• Simple Stain Procedure (Video)

Unit 5

• Preparing a Slide with Two Organisms (Replaces Two Specimen Slide) (Video)
• Gram Stain (Video)

Unit 6

Unit 7

• Chocolate Agar (Video)
• TSA with Blood (Video)
• MacConkey's (Video)
  

Unit 8

• Catalase Test (Video)
• Oxidase Test (Video)
• Evaluating Streak Isolation Results (Video)

Unit 9

• Triple Sugar Iron Slant (Video)
• MR/VP Broth (Video)
• Carbohydrate Fermentation Durham Tubes (Video)

Unit 10

• Lysine (Video)
• Phenylalanine Deaminase (Video)
• SIM (Video)
• Urease Test (Video)

Unit 11

• Catalase Test (Video)
• Coagulase (Video)

Unit 12

• Novobiocin (Video)
• A/P Disks (Video) (Note: This video uses a CNA blood plate but we will use the TSA with blood.)